1 Overview of Microscopical Approaches
1.1 Introduction
1.2 Light microscopy
1.2.1 Compound light microscope
Dark field and phase contrast
Polarised light and differential interference contrast
Fluorescent microscopy
Staining
1.2.2 Confocal scanning light microscopy
1.2.3 Specimen preparation
1.3 Electron microscopy
1.3.1 Scanning electron microscopy
1.3.2 Transmission electron microscopy
Thin section[...]
1 Overview of Microscopical Approaches
1.1 Introduction
1.2 Light microscopy
1.2.1 Compound light microscope
Dark field and phase contrast
Polarised light and differential interference contrast
Fluorescent microscopy
Staining
1.2.2 Confocal scanning light microscopy
1.2.3 Specimen preparation
1.3 Electron microscopy
1.3.1 Scanning electron microscopy
1.3.2 Transmission electron microscopy
Thin sectioning
Replica techniques
Dispersions
1.4 Other techniques
1.5 Conclusions
Bibliography
2 Instrumental Techniques for Sample Preparation
2.1 Introduction
2.2 Light microscopy techniques
2.2.1 Wide-field light microscopy
2.2.2 Fluorescence light microscopy
2.2.3 Confocal scanning laser microscopy
2.2.4 Sectioned material for light microscopy
2.2.5 Cold-stage light microscopy
2.3 Scanning electron microscopy
2.3.1 Electron guns
2.3.2 Cathode comparison parameters
2.3.3 Low-temperature scanning electron microscopy
2.3.4 Environmental/variable pressure scanning electron microscopy
2.4 Transmission electron microscopy
2.4.1 Embedded material for transmission electron microscopy
2.4.2 Negative staining transmission electron microscopy
2.4.3 Low-temperature transmission electron microscopy
Freeze-fracture replication
Low-temperature preparation methods
Freeze-substitution for transmission electron microscopy
Cryo-transmission electron microscopy
2.4.4 Energy-filtering transmission electron microscopy techniques
Global imaging
Contrast enhancement
Thick section imaging
Frozen hydrated specimens
Electron spectroscopic imaging
2.5 X-ray microanalysis
2.6 Rheology
2.7 Light scattering
2.7.1 Laser light scattering
2.7.2 Dynamic light scattering
2.8 Nuclear magnetic resonance spectroscopy
2.9 Digital imaging and image analysis
2.9.1 Hardware
2.9.2 Software
2.9.3 Major steps in applying image analysis
Image acquisition
Calibration
Image enhancement
2.10 Laboratory safety
2.10.1 Light microscopy
2.10.2 Scanning electron microscopy/transmission electron microscopy
2.10.3 Systems using lasers
2.11 Future techniques in dairy product structure
2.11.1 Scanning probe microscopy
2.11.2 Diffusing wave and ultrasonic spectroscopy
2.11.3 Microwave techniques in microscopy
References
3 Microstructure of Milk Components
3.1 Introduction
3.2 Cow’s milk composition
3.2.1 Milk fat globule
Milk fat
Milk fat globule membrane
3.2.2 Colloidal milk proteins
Microstructure of casein micelles
Molecular structure of casein micelles
Interactions and stabilisation of casein micelles
3.2.3 Whey proteins
3.2.4 Lactose
3.3 Concluding remarks
References
4 Microstructure of Dairy Fat Products
4.1 Introduction
4.2 Microstructure of cream and butter
4.2.1 Background
4.2.2 Cream
4.2.3 Whipped cream
4.2.4 Butter
4.3 Milk fat
4.3.1 Composition
4.3.2 Fractionation
4.4 Microstructure
4.4.1 Effect of minor components
4.4.2 Processing conditions
Crystallisation temperature
Cooling rate
Agitation
4.4.3 Chemical composition
Interesterification
Blending anhydrous milk fat with vegetable oils
Blending different anhydrous milk fat fractions
Blending anhydrous milk fat with cocoa butter
4.4.4 Addition of emulsifiers to milk fat and its blends
4.5 Rheology
4.6 Fat crystal networks and microstructure
4.7 Milk fat in emulsions
4.7.1 Introduction
4.7.2 Emulsion stability
Creaming
Flocculation
Coalescence
Partial coalescence
Ostwald ripening
4.8 Conclusions
Acknowledgements
References
5 Microstructure of Concentrated and Dried Milk Products
5.1 Introduction
5.2 Patterns of production
5.3 Milk as a raw material
5.4 Concentrated milk products
5.4.1 Evaporated milk
Method of manufacture
Structure
5.4.2 Sweetened condensed milk
Method of manufacture
Structure
5.4.3 Bulk concentrated milk
5.5 Dairy powders
5.5.1 Whole and skimmed milk powders
Method of manufacture
Structure
5.5.2 Retentate milk (whole and skimmed) powders
Method of manufacture
Structure
5.5.3 Buttermilk powder
Method of manufacture
Structure
5.5.4 Whey powder
Method of manufacture
Structure
5.5.5 Whey protein concentrates and whey protein isolates
Method of manufacture
Structure
5.5.6 Lactose
Method of manufacture
Structure
5.5.7 Caseinates
Method of manufacture
Structure
5.6 Conclusion References
6 Structure of Fermented Milks
6.1 Introduction
6.2 Background to manufacturing practice
6.3 Patterns of production and consumption
6.4 Lactic acid fermentations
6.4.1 Background
6.4.2 Liquid-type fermented milks
Ayran and dough
Buttermilk
Yakult@
6.4.3 Gel-type fermented milks
Factors affecting the structure formation in yoghurt
Monitoring milk coagulation using confocal scanning laser microscopy
Effect of capsular EPS on structure formation and origin of void spaces around starter cultures
How is ropiness formed?
Stabilising mechanism of EPS in yoghurt
6.5 Lactic acid-yeast fermentations
6.5.1 Kefir
Structure
6.5.2 Koumiss
6.5.3 Calpis@
6.6 Lactic acid-mould fermentations
6.6.1 Viili (viiliä)
6.7 Concentrated fermented milk products
6.7.1 Labneh
6.7.2 Skyr
6.7.3 Ymer
6.7.4 Shrikhand
6.8 Frozen yoghurt and related products
6.9 Dried fermented milk products
6.10 Conclusions
References
7 Microstructure of Natural Cheeses
7.1 Introduction
7.2 Manufacturing processes
7.2.1 High-pressure processing
7.2.2 Cheese manufactured from concentrated milk
UItrafiltered milk
Evaporated milk
7.2.3 Freezing
7.3 Compositional parameters
7.3.1 Calcium and pH
7.3.2 Salt
7.3.3 Fat replacers
7.3.4 Milk from different mammalian species
7.4 Fat globule microstructure
7.4.1 Homogenisation
7.4.2 Recombined milk
7.4.3 Free oil formation
7.4.4 Meltability
7.5 Protein microstructure
7.5.1 Protein hydrolysis
7.5.2 Casein substructural units
7.6 MineraIs
7.7 Mozzarella and other pasta filata cheeses
7.7.1 Microstructure of Mozzarella cheese
7.7.2 Protein microstructure
7.7.3 Compositional parameters
Calcium and pH
Salt
Exopolysaccharides
Fat replacers
7.7.4 Fat globule microstructure and meltability
7.7.5 Homogenisation
7.7.6 Recombined milk
7.7.7 Light scattering by cheese constituents
7.8 Cottage cheeses
7.9 Cream cheeses
7.10 Mould-ripened cheeses
7.10.1 Background
7.10.2 Camembert cheese
7.11 Conclusions
References
8 Processed Cheese and Cheese Analogues
8.1 Introduction
8.2 Processed cheese technology
8.2.1 Gross composition and ingredients
8.2.2 Emulsifying salts
8.2.3 Manufacturing process
8.3 Rheology and structure of processed cheese
8.3.1 Background
8.3.2 Influence of blending ingredients
8.3.3 Effects of moisture and pH
8.3.4 Processing conditions
8.3.5 Changes during cooling and storage
8.4 Conclusion
References
9 Microstructure of Frozen and Dairy-Based Confectionery Products
9.1 Introduction
9.2 Technological issues
9.2.1 Ice cream
9.2.2 Chocolate and couvertures
9.2.3 Caramel and toffee
9.3 Microscopical issues
9.3.1 Ice cream
9.3.2 Toffee formulations
9.3.3 Chocolate and couvertures
9.4 Conclusions
References
10 The Microscope in Troubleshooting
10.1 Introduction
10.2 Extraneous matter
Metal checklist
Wood checklist
GIass checklist
Rubber and plastics checklist
Hair and fibres checklist
Extraneous vegetable matter checklist
AnimaIs or insects
10.3 Quasi foreign materials associated with specific products
10.4 Processing plant defects
10.5 Technological issues
10.6 Conclusions
References
Bibliography
Index
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