| Titre : | The use of fluorogenic substrates to measure fungal presence and activity in soil | | Type de document : | texte imprimé | | Auteurs : | Morten Miller, Auteur ; Ansa Palojarvi, Auteur ; Andrea Rangger, Auteur ; Morten Reeslev, Auteur ; Annelise Kjoller, Auteur | | Editeur : | Washington : American Society for Microbiology | | Année de publication : | 1998 | | Importance : | pp. 613-617 | | Présentation : | graph., tabl., réf. | | Note générale : | Vol.64 - n°2 | | Langues : | Anglais (eng) | | Catégories : | Thésaurus Agrovoc
Champignon ; Enzyme ; Corrélation génétique ; Cellulase ; Thallophyta
| | Résumé : | Our objective was to determine if 4-methylumbelliferyl-labelled enzyme substrates could be used to detect and quantify specific components of chitinase and cellulase activities as specific indicators of the presence and activity of fungal biomass. The fluorogenic substrates 4-methylumbelliferyl (MUF) N-acetyl-β-D-glucosaminide and MUF β-D-lactoside were used for the detection and quantification of β-N-acetylglucosaminidase (EC 3.2.1.30) (NAGase) and endo 1,4-β-glucanase (EC 3.2.1.4) /cellobiohydrolase (EC 3.2.1.91) (CELase), respectively. Culture screenings on solid media showed a widespread ability to produce NAGase among a taxonomically diverse selection of fungi on media with and without added chitin. NAGase activity was expressed only in a limited number of bacteria and on media supplemented with chitin. The CELase activity was observed only in a limited number of fungi and bacteria. Bacterial CELase activity was expressed on agar media containing a cellulose-derived substrate. In soil samples, NAGase activity was significantly correlated with estimates of fungal biomass, based on the content of two fungus-specific indicator molecules, 18:2ω6 phospholipid fatty acid (PLFA) and ergosterol. CELase activity was significantly correlated with the PLFA-based estimate of fungal biomass in the soil, but no correlation was found with ergosterol-based estimates of fungal biomass. | | Type de document : | Tiré à part | | Permalien de la notice : | https://infodoc.agroparistech.fr/index.php?lvl=notice_display&id=183378 |
The use of fluorogenic substrates to measure fungal presence and activity in soil [texte imprimé] / Morten Miller, Auteur ; Ansa Palojarvi, Auteur ; Andrea Rangger, Auteur ; Morten Reeslev, Auteur ; Annelise Kjoller, Auteur . - Washington : American Society for Microbiology, 1998 . - pp. 613-617 : graph., tabl., réf. Vol.64 - n°2 Langues : Anglais ( eng) | Catégories : | Thésaurus Agrovoc
Champignon ; Enzyme ; Corrélation génétique ; Cellulase ; Thallophyta
| | Résumé : | Our objective was to determine if 4-methylumbelliferyl-labelled enzyme substrates could be used to detect and quantify specific components of chitinase and cellulase activities as specific indicators of the presence and activity of fungal biomass. The fluorogenic substrates 4-methylumbelliferyl (MUF) N-acetyl-β-D-glucosaminide and MUF β-D-lactoside were used for the detection and quantification of β-N-acetylglucosaminidase (EC 3.2.1.30) (NAGase) and endo 1,4-β-glucanase (EC 3.2.1.4) /cellobiohydrolase (EC 3.2.1.91) (CELase), respectively. Culture screenings on solid media showed a widespread ability to produce NAGase among a taxonomically diverse selection of fungi on media with and without added chitin. NAGase activity was expressed only in a limited number of bacteria and on media supplemented with chitin. The CELase activity was observed only in a limited number of fungi and bacteria. Bacterial CELase activity was expressed on agar media containing a cellulose-derived substrate. In soil samples, NAGase activity was significantly correlated with estimates of fungal biomass, based on the content of two fungus-specific indicator molecules, 18:2ω6 phospholipid fatty acid (PLFA) and ergosterol. CELase activity was significantly correlated with the PLFA-based estimate of fungal biomass in the soil, but no correlation was found with ergosterol-based estimates of fungal biomass. | | Type de document : | Tiré à part | | Permalien de la notice : | https://infodoc.agroparistech.fr/index.php?lvl=notice_display&id=183378 |
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